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JIO_OK ("jump if I/O OK") tests whether CPL ≤ IOPL. The same check gates PUSHF, POPF, INT n, and IRET. The monitor then emulates each instruction as appropriate: maintaining a virtual interrupt flag per V86 task, reflecting software interrupts through the real-mode interrupt vector table, virtualizing I/O accesses, and so on.
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Segmentation maps a logical address (a 16-bit selector plus a 32-bit offset) to a 32-bit linear address, enforcing privilege and limit checks along the way. Paging then translates that linear address to a physical address, adding a second layer of User/Supervisor and Read/Write protection. The two layers are independent: segmentation is always active in protected mode, while paging is optional (controlled by CR0.PG).